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KMID : 0380020130280020115
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 2 p.115 ~ p.122
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Comparison of Volatile Compounds of Chamaecyparis obtusa Essential Oil and its Application on the Improvement of Atopic Dermatitis
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Lim Geum-Sook
Kim Ran
Cho Hoon
Moon Young-Sook
Choi Chang-Nam
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Abstract
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Volatile flavor compounds of Chamaecyparis obtuse essential oil were extracted by simultaneous steam distillation extraction (SDE) and supercritical fluid extraction (SFE) and analyzed by GC-MS. A total of 48 and 50 components were identified in essential oil by SDE and SFE, respectively. Monoterpenes, oxygenated monoterpenes, sesquiterpenes, oxygenated sesquiterpenes, and diterpenes in essential oil by SDE were 37.24, 10.9, 9.61, 0.22, and 0.22%, respectively. In the case of SFE, they were 19.1, 23.3, 22.66, 1.31, and 10.57%, respectively. Antioxidant activities were increased with the increase of essential oil up to 80 μL/mL, irrespective of extraction method. Especially, when the essential oil concentration extracted by SDE was increased from 20 to 80 μL/mL, the antioxidant activity was increased from 10.5 to 55.1%. However, over 80 μL/mL of essential oil, an equilibrium state was maintained. In the case of essential oil extracted by SFE, it was decreased compared to that of SDE. For the improvement of atopic dermatitis, various cosmetics such as an ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE were tested. About over 90% was useful for the improvement of atopic dermatitis after 4 weeks of clinical trial targeting 40 female adults. These results demonstrate that ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE could be used in functional cosmetics.
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KEYWORD
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Chamaecyparis obtuse, Essential Oil, Simultaneous Steam Distillation Extraction, Supercritical Fluid Extraction, Antioxidant Activity, Functional Cosmetics
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